GALLOIS Jean Luc

Research Topic

I first worked on various aspects of plant development in Arabidopsis thaliana (chloroplast / meristem / gametophyte). Then, in 2008, I moved to Avignon to join the Resistance to Virus team at INRAE.  I am now leading the  ReDD team with Véronique Lefebvre.

Our research is focussed on developping genetic resistances to viruses in plants.

To successfully infect plants, viruses have to hijack host factors. Those can be turned into genetic resistances by making them unavailable to the pathogens. We aim at characterizing plant susceptibility factors in plants and develop genetic resistances to viruses of agricultural importance. We are particularly interested in assessing how genome editing technologies can be used to copy mutations across species and develop resistances.

Training and carreer

1996-2000, PhD, Joseph Fourier-Grenoble I University

2000-2003, postdoc, John Innes Centre (Norwich, UK) 

2005-2007, Research Scientist INRA-IJPB (Versailles, France) 

2000-2003 and 2008-onward. Postdoc then Research Scientist INRAE-GAFL (Avignon, France)

2014 Habilitation à Diriger les Recherches (HDR, Aix-Marseille University)

Publications/chapitres/poster

https://hal.inrae.fr/search/index/?q=jean-luc-gallois

https://orcid.org/0000-0003-0451-1740

Selected publications

Kuroiwa, K., Thenault, C., Nogué, F., Perrot, L., Mazier, M.,  Gallois, J.-L. (2022) CRISPR-based knock-out of eIF4E2 in a cherry tomato background successfully recapitulates resistance to pepper veinal mottle virus. Plant Science, Volume 316, https://doi.org/10.1016/j.plantsci.2021.111160

Zafirov, D, Giovinazzo, N, Bastet, A, Gallois, J‐L. (2021) When a knockout is an Achilles’ heel: Resistance to one potyvirus species triggers hypersusceptibility to another one in Arabidopsis thaliana. Mol Plant Pathol. https://doi.org/10.1111/mpp.13031

Bastet A, Zafirov D, Giovinazzo N, Guyon-Debast A, Nogué F, Robaglia C, Gallois JL. (2019) Mimicking natural polymorphism in eIF4E by CRISPR-Cas9 base editing is associated with resistance to potyviruses. Plant Biotechnol J. 17(9):1736-1750. https://doi.org/10.1111/pbi.13096.

Bastet, A., Robaglia, C. and Gallois, J.L. (2017) eIF4E Resistance: Natural Variation Should Guide Gene Editing. Trends Plant Sci, 22, 411-419. https://doi.org/10.1016/j.tplants.2017.01.008

Gauffier C, Lebaron C, Moretti A, Constant C, Moquet F, Bonnet G, Caranta C, Gallois JL. (2016) A TILLING approach to generate broad-spectrum resistance to potyviruses in tomato is hampered by eIF4E gene redundancy. Plant J. 85(6):717-29. https://doi.org/10.1111/tpj.13136

Older publications

Gallois, J.L.*, Guyon-Debast, A*., Lécureuil, A., Vezon, D., Carpentier, V., Bonhomme, S. and Guerche, P. (2009) The arabidopsis proteasome RPT5 subunits are essential for gametophyte development. Plant Cell 21: 442-59 https://doi.org/10.1105/tpc.108.062372

Gallois, J.-L., Nora, F.R., Mizukami, Y. et Sablowski, R. (2004) WUSCHEL induces shoot stem cell activity and developmental plasticity in the root meristem. Genes & Development18:375-380. http://genesdev.cshlp.org/content/18/4/375.full

Gallois, J.L.*, Woodward, C*, Reddy, G.V. et Sablowski, R. (2002). Combined SHOOT MERISTEMLESS and WUSCHEL trigger ectopic organogenesis in Arabidopsis. Development129:3207-3217 https://doi.org/10.1242/dev.129.13.3207